The Ypsilanti room contains the history of the city of Ypsilanti. The exhibits include:
- Ancient Indian Artifacts from the Paleo Indian era, dating from 10,000 to 8,000 B.C. - From the collection of Jacob Thumm
- War Relics
- Civil War
- Spanish-American War
- 1st and 2nd World Wars
- The Tucker Automobile
- Elijah McCoy
- The Interurban (Ypsi-Ann)
- Early Medical Practices / Instruments
- The Ypsilanti Police and Fire Fighters
- Early Businesses:
- Underware Factory
- Mineral Baths and Wells
- Paper and Flour Mills
- Soap Manufacturing
- Ginger Ale and Beer
- Schools from Kindergarten to College